https://www.stevenshuken.com/science daily 0.75 2026-03-12 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/35a5c707-882e-47c6-83c9-bbc748de690d/Screen+Shot+2023-04-29+at+9.07.20+PM.png Science - Overview - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1661964359310-LBKX0525MQVTIBKIQAR5/Screen+Shot+2022-08-31+at+6.45.09+PM.png Science - Overview - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1661964524109-UI7SI84XPGOM8EE7P2HB/Screen+Shot+2022-08-31+at+6.48.05+PM.png Science - Overview - Make it stand out A depiction of protein grouping, an essential step in a typical mass spec proteomics experiment. https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1596503573099-JAT3QC5WM6KRKH0CB6CG/Fig1_8Artboard+1.png Science - Overview https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1596504414095-RJCZX7EMU1QDROAHYH4Y/Assay+screenshot.png Science - Overview A water-soluble derivative of a hydrazine sensor allows us to measure the speeds at which hydrazine crosses different lipid membranes. https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1596505207215-Q6YHZQWL476N1ZREHJJP/Screen+Shot+2020-08-03+at+6.39.26+PM.png Science - Overview https://www.stevenshuken.com/home daily 1.0 2026-03-12 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/b0a03556-19f0-4077-a055-65e7d34d0df6/230423_headshot_399KB.png Who is Steven Shuken? Why would he make a website of himself? An artist’s rendering of Steven Shuken. https://www.stevenshuken.com/wysscoray-lab daily 0.75 2022-08-31 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1596503885456-UKIORGPLHTT7CBYLT57C/Fig1_8Artboard+1.png Wyss-Coray Lab - Studies on brain aging and Alzheimer's disease using mass spectrometry, organic synthesis, and biostatistics. That was the title of my PhD thesis, which you can download from Stanford SearchWorks. https://www.stevenshuken.com/burns-lab daily 0.75 2022-08-31 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1596505586100-XFS7X45M58E6KOBXUGA2/Assay+screenshot.png Burns Lab - Biophysical Studies on Noncanonical Membrane Lipids Using Synthetic Chemical Tools (2014–2017) Download my Master’s thesis below. More information can be found in the publications and patents, which can be accessed via my Google Scholar profile. https://www.stevenshuken.com/undergraduate-research daily 0.75 2023-12-17 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/89c6b481-b5cb-4e0a-9582-d749dcec61fb/Screen+Shot+2023-02-14+at+7.36.40+PM.png Undergraduate Research - Sarpong Group (UC Berkeley, 2012 - 2013) In the Sarpong Group I worked on an enantioselective synthesis of the enantiomer of the natural product complanadine A, which is known to stimulate neurite outgrowth in vitro. This synthesis would have helped assign the absolute configuration of complanadine A, which was disputed at the time. Enantiomers are also helpful tools for discerning whether a drug-target interaction is specific or nonspecific. In the end, I completed a formal synthesis of the enantiomer, i.e., I synthesized a starting material that is known to be used to synthesize the final product. https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1661965850339-YHB16J59YZPNT7Q0TAFD/Screen+Shot+2022-08-31+at+7.10.34+PM.png Undergraduate Research - Reisman Group (Caltech, 2013) In the Reisman Group, I synthesized bisoxazoline (BOX) ligands for nickel-catalyzed reductive asymmetric cross-coupling reactions. I completed a study of how the bite angle of the BOX ligand affects yield and enantioselectivity in three different reductive cross-coupling reactions. https://www.stevenshuken.com/simplycorrect daily 0.75 2024-02-23 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/1661939748488-TIQLMRYXXEML5DW55H5O/UncorrectedVolcanoPlots.png SIMPLYCORRECT - This is a collection of volcano plots with uncorrected or unspecified p-values that I have encountered in the wild. Uncorrected hits can be found anywhere: lab meeting presentations, conference talks or posters, submitted manuscripts, or published papers. How and why do uncorrected p-values contribute to the propagation of false knowledge? https://www.stevenshuken.com/gygi-lab daily 0.75 2024-02-23 https://images.squarespace-cdn.com/content/v1/5f02f3b0ad0f7674bd06666c/5910051e-8485-4223-8939-cb70f1f0e809/Screen+Shot+2023-04-29+at+9.07.20+PM.png Gygi Lab - Traditionally, targeted proteomics is labor- and time-intensive and has limited success rates and sample throughput. In the Gygi Lab, I am solving these problems with new computational tools and applying the resulting technology to high-throughput studies of drug-protein interactions and intracellular signaling pathways. The figure on the right is from the Gygi Lab's 2023 paper describing GoDig, a new method for targeting whole pathways of proteins with tandem mass tags (TMT) for high sample throughput. I have recently increased the success rates of GoDig to the point where single-peptide sites of interest, such as proteolytic cleavage sites, phosphorylation sites, or reactive cysteines can be reliably and sensitively quantified. I am using AI to develop methods that do not require the use of data libraries, making targeted proteomics as easy and reliable as untargeted proteomics. Yu, Q.; Liu, X.; Keller, M. P.; Navarrete-Perea, J.; Zhang, T.; Fu, S.; Vaites, L. P.; Shuken, S. R.; et al. “Sample multiplexing-based targeted pathway proteomics with real-time analytics reveals the impact of genetic variation on protein expression.” Nature Comm. 2023, 14, 555. https://www.stevenshuken.com/future-directions daily 0.75 2023-12-17 https://www.stevenshuken.com/intro-to-proteomics daily 0.75 2023-12-17